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Your Position: Home > Protein > LAG-3 > LA3-H82F3

Biotinylated Human LAG-3 / CD223 Protein, Mouse IgG2a Fc,Avitag™ (MALS verified)

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  • Synonym
    LAG3,CD223,FDC
  • Source
    Biotinylated Human LAG-3, Mouse IgG2a Fc,Avitag(LA3-H82F3) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1 ).
    Predicted N-terminus: Leu 23
  • Molecular Characterization
    LAG-3 Structure

    This protein carries a mouse IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 75.4 kDa. The protein migrates as 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
LAG-3 SDS-PAGE

Biotinylated Human LAG-3, Mouse IgG2a Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
LAG-3 MALS images

The purity of Biotinylated Human LAG-3, Mouse IgG2a Fc,Avitag (Cat. No. LA3-H82F3) is more than 90% and the molecular weight of this protein is around 170-200 kDa verified by SEC-MALS.

Bioactivity-ELISA
 LAG-3 ELISA

Immobilized Madarex LAG-3 MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human LAG-3, Mouse IgG2a Fc,Avitag (Cat. No. LA3-H82F3) with a linear range of 0.4-13 ng/mL (QC tested).

Bioactivity-FACS
 LAG-3 FACS

Flow Cytometry assay shows that Biotinylated Human LAG-3, Mouse IgG2a Fc,Avitag (Cat. No. LA3-H82F3) can bind to daudi cell surface. The concentration of LAG-3 used is 0.3 μg/mL (Routinely tested).

  • Background
    Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, which belongs to immunoglobulin (Ig) superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. LAG3 /CD223 involved in lymphocyte activation. LAG3 /CD223 binds to HLA class-II antigens.
  • Clinical and Translational Updates

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