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Your Position: Home > Immune Checkpoint Proteins > LAG-3 > Biotinylated Human LAG-3 / CD223 Protein, Mouse IgG2a Fc Tag, Avi Tag

Biotinylated Human LAG-3 / CD223 Protein, Mouse IgG2a Fc Tag, Avi Tag (Avitag™)

  • Synonym
    LAG3,CD223,FDC
  • Source
    Biotinylated Human LAG-3, Mouse IgG2a Fc Tag, Avi Tag (LA3-H82F3) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1).
    Predicted N-terminus: Leu 23
  • Molecular Characterization
    LAG-3(Leu 23 - Leu 450) P18627-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 75.4 kDa. The protein migrates as 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human LAG-3, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. LA3-H82F3) SDS-PAGE gel

Biotinylated Human LAG-3, Mouse IgG2a Fc Tag, Avi Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human LAG-3, Mouse IgG2a Fc Tag, Avi TagBiotinylated Human LAG-3, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. LA3-H82F3) ELISA bioactivity

Immobilized Madarex LAG-3 Mab,Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human LAG-3, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. LA3-H82F3) with a linear range of 0.4-13 ng/mL (QC tested).

FACS assay shows that recombinant Biotinylated FACS

Flow Cytometry assay shows that Biotinylated Human LAG-3, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. LA3-H82F3) can bind to daudi cell surface. The concentration of  LAG-3 used is 0.3 μg/ml (Routinely tested).

  • Background
    Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, which belongs to immunoglobulin (Ig) superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. LAG3 /CD223 involved in lymphocyte activation. LAG3 /CD223 binds to HLA class-II antigens.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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