>Immune Checkpoint Proteins
>Biotinylated Human LAG-3 / CD223 Protein, Mouse IgG2a Fc Tag, Avi Tag
This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 75.4 kDa. The protein migrates as 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human LAG-3, Mouse IgG2a Fc Tag, Avi Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Madarex LAG-3 Mab,Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human LAG-3, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. LA3-H82F3) with a linear range of 0.4-13 ng/mL (QC tested).
Flow Cytometry assay shows that Biotinylated Human LAG-3, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. LA3-H82F3) can bind to daudi cell surface. The concentration of LAG-3 used is 0.3 μg/ml (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $350.00
Price(USD) : $1380.00
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