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Your Position: Home > Immune Checkpoint Proteins > B7-H3 > B73-H82F5

Biotinylated Human B7-H3 / CD276 Protein, Fc,Avitag™ (MALS verified)

  • Synonym
    B7-H3,CD276,B7 homolog 3
  • Source
    Biotinylated Human B7-H3, Fc,Avitag (B73-H82F5) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Pro 245 (Accession # Q5ZPR3-2).
    Predicted N-terminus: Leu 29
  • Molecular Characterization
    Online(Leu 29 - Pro 245) Q5ZPR3-2

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 51.6 kDa. The protein migrates as 63-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human B7-H3, Fc,Avitag (Cat. No. B73-H82F5) SDS-PAGE gel

Biotinylated Human B7-H3, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human B7-H3, Fc,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human B7-H3, Fc,Avitag (Cat. No. B73-H82F5) was more than 90% and the molecular weight of this protein is around 115-135 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human B7-H3, Fc,AvitagBiotinylated Human B7-H3, Fc,Avitag (Cat. No. B73-H82F5) ELISA bioactivity

Immobilized Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human B7-H3, Fc,Avitag (Cat. No. B73-H82F5) with a linear range of 0.3-2 ng/mL (QC tested).

Biotinylated Human B7-H3, Fc,AvitagBiotinylated Human B7-H3, Fc,Avitag (Cat. No. B73-H82F5) ELISA bioactivity

Immobilized Biotinylated Human B7-H3, Fc,Avitag (Cat. No. B73-H82F5) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (Routinely tested).

  • Background
    B7 homolog 3 (B7-H3), a member of the immunoglobulin superfamily, is also known CD276, which contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domains. B7-H3 may participate in the regulation of T-cell-mediated immune response. B7-H3 also plays a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. Furthermore, B7-H3 is involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. It could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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