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Product Details
Synonyms
RSPO1, CRISTIN3, FLJ40906, RP11-566C13.1, R-spondin-1
Source
Human R-Spondin 1, His Tag (RS1-H4221) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Ala 263 (Accession # Q2MKA7-1).
Predicted N-terminus: Ser 21
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 28.7 kDa. The protein migrates as 38-42 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human R-Spondin 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human R-Spondin 1, His Tag (Cat. No. RS1-H4221) at 5 μg/mL (100 μL/well) can bind Human SCARB2, Fc Tag (Cat. No. SC2-H5250) with a linear range of 0.02-1.25 μg/mL (QC tested).
Protocol
Immobilized Human R-Spondin 1, His Tag (Cat. No. RS1-H4221) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD36, His,Avitag (Cat. No. CD6-H82E9) with a linear range of 0.01-1.25 μg/mL (Routinely tested).
Protocol
Immobilized Human R-Spondin 1, His Tag (Cat. No. RS1-H4221) at 5 μg/mL (100 μL/well) can bind Human LRP-6 (20-630), Mouse IgG2a Fc Tag (Cat. No. LR6-H5253) with a linear range of 1-20 ng/mL (Routinely tested).
Protocol
Bioactivity-CELL BASE
Human R-Spondin 1, His Tag (Cat. No. RS1-H4221) induced TCF reporter activity in HEK293 cells. The EC50 for this effect is 0.0035-0.0040 µg/mL (Routinely tested).
Protocol
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