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Your Position: Home > All Other Proteins > CD30 > Human CD30 / TNFRSF8 Protein, Llama IgG2b Fc Tag, low endotoxin

Human CD30 / TNFRSF8 Protein, Llama IgG2b Fc Tag, low endotoxin

  • Synonym
    TNFRSF8,CD30,D1S166E,Ki-1
  • Source
    Human CD30, Llama IgG2b Fc Tag, low endotoxin (TN8-H5250) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Lys 379 (Accession # NP_001234.2).
    Predicted N-terminus: Phe 19
  • Molecular Characterization
    CD30(Phe 19 - Lys 379) NP_001234.2

    This protein carries a llama IgG2b Fc tag at the C-terminus.

    The protein has a calculated MW of 66.3 kDa. The protein migrates as 95-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD30, Llama IgG2b Fc Tag, low endotoxin (Cat. No. TN8-H5250) SDS-PAGE gel

Human CD30, Llama IgG2b Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human CD30, Llama IgG2b Fc Tag, low endotoxinHuman CD30, Llama IgG2b Fc Tag, low endotoxin (Cat. No. TN8-H5250) ELISA bioactivity

Immobilized Human CD30, Llama IgG2b Fc Tag, low endotoxin (Cat. No. TN8-H5250) at 0.5 μg/mL (100 μL/well) can bind Anti-CD30 Mab, Human IgG1 with a linear range of 0.1-1.6 ng/mL (Routinely tested).

Human CD30, Llama IgG2b Fc Tag, low endotoxinHuman CD30, Llama IgG2b Fc Tag, low endotoxin (Cat. No. TN8-H5250) ELISA bioactivity

Immobilized Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525b) at 0.5 μg/mL (100 μL/well) can bind Human CD30, Llama IgG2b Fc Tag, low endotoxin (Cat. No. TN8-H5250) with a linear range of 10-78 ng/mL (QC tested).

Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.

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