This protein carries no "tag".
The protein has a calculated MW of 17.4 kDa. The protein migrates as 17 kDa under reducing (R) condition (SDS-PAGE).
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human TNF-alpha, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human TNF-alpha, premium grade (Cat. No. TNA-H4211) is more than 95% and the molecular weight of this protein is around 47-60 kDa verified by SEC-MALS.
Human TNF-alpha, premium grade (Cat. No. TNA-H4211) induces cytotoxicity effect on the WEHI-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The specific activity of Human TNF-alpha, premium grade is ≥ 2.0×10^7 IU/mg, which is calibrated against human TNF-alpha WHO International Standard (NIBSC code: 12/154) (QC tested).
Activity of three different production batches of Human TNF-alpha, premium grade (Cat. No. TNA-H4211).
Neutralization assay shows that the cytotoxicity effect of Human TNF-alpha, premium grade (Cat. No. TNA-H4211) was inhibited by increasing concentration of Adalimumab. The concentration of TNF-alpha used is 1 ng/mL. The IC50 is 29 ng/mL (Routinely tested).
The NF-kB (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of Human TNF-alpha, premium grade (Cat. No. TNA-H4211). The max induction fold was approximately 235 (Routinely tested).
The activity of Human TNF-alpha, premium grade (TNA-H4211) was higher than other competing products.
The Cell based assay shows that Human TNF-alpha, premium grade (TNA-H4211) is stable at 37°C for 48 hours.
The Cell based assay shows that Human TNF-alpha, premium grade (TNA-H4211) is stable after freezing and thawing 3 times.
Immobilized Human TNF-alpha, premium grade (Cat. No. TNA-H4211) at 2 μg/mL (100 μL/well) can bind Humira with a linear range of 0.2-2 ng/mL (QC tested).
Immobilized Human TNF-alpha, premium grade (Cat. No. TNA-H4211) at 2 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag (Cat. No. TN1-H5251) with a linear range of 0.2-3 ng/mL (Routinely tested).
Immobilized Human TNF-alpha, premium grade (Cat. No. TNA-H4211) at 5 μg/mL (100 μL/well) can bind Human TNFR2, His Tag (Cat. No. TN2-H5227) with a linear range of 5-39 ng/mL (Routinely tested).
Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha, premium grade (Cat. No. TNA-H4211) with an affinity constant of 0.255 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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