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Your Position: Home > All Other Proteins > RANK > RAK-H5251

Human RANK / TNFRSF11A Protein, Mouse IgG2a Fc Tag, low endotoxin (MALS verified)

  • Synonym
    CD265,FEO,LOH18CR1,ODFR,OFE,OPTB7,OSTS,PDB2,RANK,TNFRSF11A,TRANCER,CD265 antigen,ODFROSTS,RANKLOH18CR1,NFKB activator
  • Source
    Human RANK, Mouse IgG2a Fc Tag, low endotoxin (RAK-H5251) is expressed from human 293 cells (HEK293). It contains AA Ile 30 - Pro 212 (Accession # Q9Y6Q6-1).
    Predicted N-terminus: Ile 30
  • Molecular Characterization
    Online(Ile 30 - Pro 212) Q9Y6Q6-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 47.2 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) SDS-PAGE gel

Human RANK, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. ) MALS images

The purity of Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) was more than 85% and the molecular weight of this protein is around 115-125 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human RANK, Mouse IgG2a Fc Tag, low endotoxinHuman RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) ELISA bioactivity

Immobilized Human TNFSF11, His Tag (Cat. No. RAL-H5240) at 2 μg/mL (100 μL/well) can bind Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) with a linear range of 0.4-13 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Captured Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) on CM5 chip via Anti-Mouse antibodies surface can bind Human TNFSF11, His Tag (Cat. No. RAL-H5240) with an affinity constant of 0.309 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) on AMC Biosensor, can bind Human TNFSF11, His Tag (active trimer) (Cat. No. RAL-H5240) with an affinity constant of 0.409 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Order Details

Price(USD) : $350.00

Price(USD) : $2100.00

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Datasheet & Documentation

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DMF (Drug Master File)

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