This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 45.7 kDa. The protein migrates as 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human / Cynomolgus / Rhesus macaque ROR1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human / Cynomolgus / Rhesus macaque ROR1, His,Avitag (Cat. No. RO1-H82E6) is more than 90% and the molecular weight of this protein is around 57-67 kDa verified by SEC-MALS.
Anti-Human ROR1 mAb captured on CM5 chip via anti-mouse antibodies surface can bind Biotinylated Human / Cynomolgus / Rhesus macaque ROR1, His, Avitag (Cat. No. RO1-H82E6) with an affinity constant of 5.81 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
2e5 of anti-ROR1 CAR-293 cells were stained with 100 μL of 10 μg/mL of Biotinylated Human / Cynomolgus / Rhesus macaque ROR1, His,Avitag (Cat. No. RO1-H82E6) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
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