This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 71.2 kDa. The protein migrates as 85-100 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Cynomolgus Her2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Cynomolgus Her2, His Tag (Cat. No. HE2-C52Hb) is more than 85% and the molecular weight of this protein is around 75-105 kDa verified by SEC-MALS.
Loaded Hercecptin on AHC Biosensor, can bind Cynomolgus Her2, His Tag (Cat. No. HE2-C52Hb) with an affinity constant of 4.86 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Hussack G, et al
Journal: BMC Res Notes 2018
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