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Your Position: Home > All Other Proteins > IGF-I R > IGR-C5225

Cynomolgus IGF-I R / CD221 Protein, His Tag (MALS verified)

  • Synonym
    IGF1R,IGFR,JTK13,CD221,MGC142170,MGC142172,MGC18216
  • Source
    Cynomolgus IGF-I R, His Tag (IGR-C5225) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # G7P9I7-1).
    Predicted N-terminus: Glu 31 & Asp 741
  • Molecular Characterization
    Online(Glu 31 - Asn 932) G7P9I7-1

    This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 737-RKRR-740, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.1 kDa and 23.8 kDa respectively. The protein migrates as 45-50 kDa (partial β chain), 96-115 kDa (α chain) and 120 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus IGF-I R, His Tag (Cat. No. IGR-C5225) SDS-PAGE gel

Cynomolgus IGF-I R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Cynomolgus IGF-I R, His Tag (Cat. No. ) MALS images

The purity of Cynomolgus IGF-I R, His Tag (Cat. No. IGR-C5225) is more than 90% and the molecular weight of this protein is around 255-311 kDa verified by SEC-MALS.

Bioactivity-ELISA
Cynomolgus IGF-I R, His TagCynomolgus IGF-I R, His Tag (Cat. No. IGR-C5225) ELISA bioactivity

Immobilized Cynomolgus IGF-I R, His Tag (Cat. No. IGR-C5225) at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag (Cat. No. IG1-H4269) with a linear range of 5-78 ng/mL (QC tested).

  • Background
    The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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