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Your Position: Home > Immune Checkpoint Proteins > CD28 > CD8-H525a

Human / Cynomolgus / Rhesus macaque CD28 Protein, Fc Tag, active dimer (MALS verified)

  • Synonym
    CD28,Tp44
  • Source
    Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (CD8-H525a) is expressed from human 293 cells (HEK293). It contains AA Asn 19 - Pro 152 (Accession # P10747-1). In the region Asn 19 - Pro 152, the AA sequence of Human, Cynomolgus and Rhesus macaque CD28 are homologus.
    Predicted N-terminus: Asn 19
  • Molecular Characterization
    Online(Asn 19 - Pro 152) P10747-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 41.6 kDa. As a result of glycosylation, the protein migrates as 53-66 kDa under reducing (R) condition, and 100-120 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) SDS-PAGE gel

Human / Cynomolgus / Rhesus macaque CD28, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. ) MALS images

The purity of Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) was more than 90% and the molecular weight of this protein is around 105-125 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human / Cynomolgus / Rhesus macaque CD28, Fc TagHuman / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) ELISA bioactivity

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) at 5 μg/mL (100 μL/well) can bind Biotinylated Human B7-2, Fc Tag (Cat. No. CD6-H82F5) with a linear range of 0.019-0.625 μg/mL (QC tested).

Human / Cynomolgus / Rhesus macaque CD28, Fc TagHuman / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) ELISA bioactivity

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) at 2 μg/mL (100 μL/well) can bind Anti-Mouse CD28 MAb with a linear range of 0.1-2 ng/mL (QC tested).

Human / Cynomolgus / Rhesus macaque CD28, Fc TagHuman / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) ELISA bioactivity

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) at 5 μg/mL (100 μL/well) can bind Biotinylated Human B7-1, Fc Tag (Cat. No. B71-H82F2) with a linear range of 0.313-5 μg/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) on CM5 Chip can bind Human B7-1, His Tag (Cat. No. B71-H5228) with an affinity constant of 5.29 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Human_FcRn_Heterodimer_Protein_SPR

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) on CM5 Chip can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) with an affinity constant of 2.72 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Human_FcRn_Heterodimer_Protein_SPR

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) on CM5 Chip can bind Human B7-1, Fc Tag (Cat. No. B71-H5259) with an affinity constant of 2.01 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) on ProteinA Biosensor, can bind Human B7-1, His Tag (Cat. No. B71-H5228) with an affinity constant of 2.2 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (Cat. No. CD8-H525a) on Protein A Biosensor, can bind Human B7-2, His Tag (Cat. No. CD6-H5223) with an affinity constant of 6.8 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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