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Your Position: Home > Isotype Controls > IgG1 Fc > FCC-H5214

Human IgG1 Fc Protein, Tag Free (MALS verified)

  • Synonym
    IgG1 Fc,IgG2,IgG3,IgG4,IgG2a,IgG Fc,IgG1
  • Source
    Human IgG Fc, Tag Free (FCC-H5214) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 330 (Accession # P01857-1).
    Predicted N-terminus: Glu 99
  • Molecular Characterization
    Online(Glu 99 - Lys 330) P01857-1

    This protein carries no "tag".

    The protein has a calculated MW of 26.1 kDa. The protein migrates as 32-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Human IgG Fc, Tag Free (Cat. No. FCC-H5214) SDS-PAGE gel

Human IgG Fc, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Human IgG Fc, Tag Free (Cat. No. ) MALS images

The purity of Human IgG Fc, Tag Free (Cat. No. FCC-H5214) was more than 95% and the molecular weight of this protein is around 51-65 kDa verified by SEC-MALS.

Human IgG Fc, Tag FreeHuman IgG Fc, Tag Free (Cat. No. FCC-H5214) ELISA bioactivity

Immobilized Human CD64, His Tag (Cat. No. FCA-H52H1) at 1 μg/mL (100 μL/well) can bind Human IgG Fc, Tag Free (Cat. No. FCC-H5214) with a linear range of 1-16 ng/mL (QC tested).


Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) captured on CM5 Chip via anti-His antibody can bind Human IgG1 Fc, Tag Free (Cat. No. FCC-H5214) with an affinity constant of 0.957 μM as determined in SPR assay (Biacore 8K) (Routinely tested).

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