This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 28.5 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Cynomolgus DNAM-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Cynomolgus DNAM-1, His Tag (Cat. No. DN1-C52H9) at 2 μg/mL (100 μL/well) can bind Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) with a linear range of 2-40 ng/mL (QC tested).
Immobilized Cynomolgus DNAM-1, His Tag (Cat. No. DN1-C52H9) at 2 μg/mL (100 μL/well) can bind Human Nectin-2, Fc Tag (Cat. No. PV2-H5253) with a linear range of 5-40 ng/mL (Routinely tested).
Loaded Human CD155, Fc Tag (Cat. No. CD5-H5251) on Protein A Biosensor, can bind Cynomolgus DNAM-1, His Tag (Cat. No. DN1-C52H9) with an affinity constant of 0.88 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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