Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of ITGA4 and ITGB1, has a calculated MW of 113.2 kDa (ITGA4) and 83.7 kDa (ITGB1). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The ITGA4 subunit is composed of a heavy chain (Tyr 34 - Arg 591, calculated MW 61.1 kDa) and a light chain (Ser 592 - Thr 977, calculated MW 52.1 kDa). Consequently ITGA4 migrates as 65-75 kDa, 80-85 kDa and 135-150 kDa, and ITGB1 as 100-133 kDa respectively under reducing (R) condition due to cleavage and glycosylation.
>95% as determined by SDS-PAGE.
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Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Natalizumab at 1 μg/mL (100 μL/well) can bind Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) with a linear range of 2-31 ng/mL (QC tested).
Authors: Takada, Yoko K et al.
Journal: The Journal of biological chemistry 2021
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