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Your Position: Home > All Other Proteins > BAFFR > BAR-H5258

Human BAFFR / TNFRSF13C Protein, Llama IgG2b Fc Tag, low endotoxin

  • Synonym
    BAFFR,TNFRSF13C,BROMIX,CD268,CVID4,prolixin,BAFF-R
  • Source
    Human BAFFR, Llama IgG2b Fc Tag, low endotoxin (BAR-H5258) is expressed from human 293 cells (HEK293). It contains AA Ser 7 - Ala 71 (Accession # Q96RJ3-1).
    Predicted N-terminus: Ser 7
  • Molecular Characterization
    Online(Ser 7 - Ala 71) Q96RJ3-1

    This protein carries a llama IgG2b Fc tag at the C-terminus.

    The protein has a calculated MW of 34.4 kDa. The protein migrates as 42-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human BAFFR, Llama IgG2b Fc Tag, low endotoxin (Cat. No. BAR-H5258) SDS-PAGE gel

Human BAFFR, Llama IgG2b Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human BAFFR, Llama IgG2b Fc Tag, low endotoxinHuman BAFFR, Llama IgG2b Fc Tag, low endotoxin (Cat. No. BAR-H5258) ELISA bioactivity

Immobilized Human BAFF, His Tag (Cat. No. BAF-H5248) at 2 μg/mL (100 μL/well) can bind Human BAFFR, Llama IgG2b Fc Tag, low endotoxin (Cat. No. BAR-H5258) with a linear range of 2-63 ng/mL (QC tested).

Human BAFFR, Llama IgG2b Fc Tag, low endotoxinHuman BAFFR, Llama IgG2b Fc Tag, low endotoxin (Cat. No. BAR-H5258) ELISA bioactivity

Immobilized Human BAFFR, Llama IgG2b Fc Tag, low endotoxin (Cat. No. BAR-H5258) at 2 μg/mL (100 μL/well) can bind Biotinylated Human BAFF, His,Avitag (Cat. No. BAF-H82Q2) with a linear range of 0.2-4 ng/mL (Routinely tested).

Human BAFFR, Llama IgG2b Fc Tag, low endotoxinHuman BAFFR, Llama IgG2b Fc Tag, low endotoxin (Cat. No. BAR-H5258) ELISA bioactivity

Immobilized Human BAFFR, Llama IgG2b Fc Tag, low endotoxin (Cat. No. BAR-H5258) at 1 μg/mL (100 μL/well) can bind Anti-BAFFR Antibody, Human IgG1 with a linear range of 0.2-5 ng/mL (QC tested).

Human BAFFR, Llama IgG2b Fc Tag, low endotoxinHuman BAFFR, Llama IgG2b Fc Tag, low endotoxin (Cat. No. BAR-H5258) ELISA bioactivity

Serial dilutions of Anti-BAFFR Antibody, Human IgG1 were added into Human BAFFR, Llama IgG2b Fc Tag, low endotoxin (Cat. No. BAR-H5258): Biotinylated Human BAFF, His,Avitag (active trimer) (MALS verified) (Cat. No. BAF-H82Q2) binding reactions. The half maximal inhibitory concentration (IC50) is 1.1323 μg/mL (Routinely tested).

Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.
Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Immobilized Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human BAFFR, Llama IgG2b Fc Tag, low endotoxin (Cat. No. BAR-H5258) with an affinity constant of 44.5 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

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Order Details

Price(USD) : $350.00

Price(USD) : $2310.00

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