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Your Position: Home > Immune Checkpoint Proteins > B7-H3 (4Ig) > Human B7-H3 (4Ig) / B7-H3b Protein, Fc Tag

Human B7-H3 (4Ig) / B7-H3b Protein, Fc Tag

  • Synonym
    4Ig-B7-H3,B7-H3,CD276,PSEC0249,UNQ309/PRO352,B7 homolog 3
  • Source
    Human B7-H3 (4Ig), Fc Tag (B7B-H5258) is expressed from human 293 cells (HEK293). It contains AA Gly 27 - Thr 461 (Accession # Q5ZPR3-1).
    Predicted N-terminus: Gly 27
  • Molecular Characterization
    Online(Gly 27 - Thr 461) Q5ZPR3-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 73.1 kDa. The protein migrates as 100-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human B7-H3 (4Ig), Fc Tag (Cat. No. B7B-H5258) SDS-PAGE gel

Human B7-H3 (4Ig), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

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