This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 22.2 kDa. The protein migrates as 32-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mouse CD32b, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Mouse CD32b, His Tag (Cat. No. CDB-M52H7) is more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.
Immobilized Mouse CD32b, His Tag (Cat. No. CDB-M52H7) on NTA-Series S sensor chip, can bind OKT3 with an affinity constant of 0.86 μM as determined in a SPR assay (Biacore T200) (QC tested).
Immobilized OKT3 on Protein G-Series S sensor chip, can bind Mouse CD32b, His Tag (Cat. No. CDB-M52H7) with an affinity constant of 0.55 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Mouse CD32b, His Tag (Cat. No. CDB-M52H7) on NTA Biosensor, can bind OKT3 with an affinity constant of 1.2 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Mouse CD32b, His Tag (Cat. No. CDB-M52H7) on NTA Biosensor, can bind Herceptin with an affinity constant of 2.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded OKT3 on Protein G Biosensor, can bind Mouse CD32b, His Tag (Cat. No. CDB-M52H7) with an affinity constant of 1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Herceptin on Protein G Biosensor, can bind Mouse CD32b, His Tag (Cat. No. CDB-M52H7) with an affinity constant of 1.2 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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