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Your Position: Home > All Other Proteins > CD19 > CD9-HF2H2

FITC-Labeled Human CD19 (20-291) Protein, His Tag DMF Filed

  • Synonym
    CD19,B4,CVID3,MGC12802
  • Source
    FITC-Labeled Human CD19 (20-291), His Tag (Cat. No. CD9-HF2H2) is expressed from human HEK293 cells. It contains AA Pro 20 - Lys 291 (Accession # P15391-1). It is the FITC labeled form of Human CD19, His Tag (Cat. No. CD9-H52H2).
    Predicted N-terminus: Pro 20
  • Molecular Characterization
    Online(Pro 20 - Lys 291) P15391-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 32.0 kDa. The protein migrates as 45-55 kDa and 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
  • FITC:Protein Ratio
    The FITC to protein molar ratio is 1.5-3.5.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
FITC-Labeled Human CD19 (20-291), His Tag (Cat. No. CD9-HF2H2) SDS-PAGE gel

FITC-Labeled Human CD19 (20-291), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Evaluation of CAR expression
FACS Analysis of Anti-CD19 CAR Expression
Evaluation of CAR expression

293 cells were transfected with anti-CD19-scFv and RFP tag. 2e5 of the cells were stained with B.  FITC-Labeled Human CD19 (20-291), His Tag (Cat. No. CD9-HF2H2, 10 µg/ml) and C. FITC-labeled protein control. A. Non-transfected 293 cells and C. FITC-labeled protein control were used as negative control. RFP was used to evaluate CAR (anti-CD19-scFv) expression and FITC was used to evaluate the binding activity of  FITC-Labeled Human CD19 (20-291), His Tag (Cat. No. CD9-HF2H2) (QC tested).

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Price(USD) : $319.00

Price(USD) : $1980.00

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