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Your Position: Home > Protein > CEACAM-5 > CE5-H82E0

Biotinylated Human CEACAM-5 / CD66e Protein, His,Avitag™ (MALS verified)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.

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  • Synonym
    CEACAM-5,CD66e,CEA,Meconium antigen 100
  • Source
    Biotinylated Human CEACAM-5, His,Avitag(CE5-H82E0) is expressed from human 293 cells (HEK293). It contains AA Lys 35 - Ala 685 (Accession # P06731-1).
    Predicted N-terminus: Lys 35
  • Molecular Characterization
    CEACAM-5 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 75.0 kDa. The protein migrates as 90-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM Potassium glutamate, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CEACAM-5 SDS-PAGE

Biotinylated Human CEACAM-5, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
CEACAM-5 MALS images

The purity of Biotinylated Human CEACAM-5, His,Avitag (Cat. No. CE5-H82E0) is more than 90% and the molecular weight of this protein is around 205-220 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CEACAM-5 ELISA

Immobilized Biotinylated Human CEACAM-5, His,Avitag (Cat. No. CE5-H82E0) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate. can bind Anti-CEACAM-5 (labetuzumab) Antibody, Human IgG1 with a linear range of 0.3-10 ng/mL (QC tested).

 CEACAM-5 ELISA

Immobilized Anti-CEACAM-5 (labetuzumab) Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human CEACAM-5, His,Avitag (Cat. No. CE5-H82E0) with a linear range of 0.6-20 ng/mL (Routinely tested).

Bioactivity-SPR
 CEACAM-5 SPR

Anti-CEACAM-5 (labetuzumab) Antibody, Human IgG1 captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Biotinylated Human CEACAM-5, His,Avitag (Cat. No. CE5-H82E0) with an affinity constant of 12.1 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

  • Background
    Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) is also Carcinoembryonic antigen (CEA), Meconium antigen 100, CD antigen CD66e, CEACAM5 belongs to the immunoglobulin superfamily and CEA family. CEACAM5 contains seven Ig-like (immunoglobulin-like) domains. CEACAM5 is homodimer protein which binding of E.coli Dr adhesins leads to dissociation of the homodimer. CEACAM5 is cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEACAM5 is receptor for E.coli Dr adhesins.
  • Clinical and Translational Updates

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