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Your Position: Home > CKP > LAG-3 > LA3-H82Fb

Biotinylated Human LAG-3 / CD223 Protein, Fc,Avitag™ (MALS verified)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    LAG3,CD223,FDC
  • Source
    Biotinylated Human LAG-3, Fc,Avitag (LA3-H82Fb) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1).
    Predicted N-terminus: Leu 23
  • Molecular Characterization
    Online(Leu 23 - Leu 450) P18627-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 75.0 kDa. The protein migrates as 90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human LAG-3, Fc,Avitag (Cat. No. LA3-H82Fb) SDS-PAGE gel

Biotinylated Human LAG-3, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human LAG-3, Fc,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human LAG-3, Fc,Avitag (Cat. No. LA3-H82Fb) is more than 90% and the molecular weight of this protein is around 200-260 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human LAG-3, Fc,AvitagBiotinylated Human LAG-3, Fc,Avitag (Cat. No. LA3-H82Fb) ELISA bioactivity

Immobilized Anti-Human LAG3 MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human LAG-3, Fc,Avitag (Cat. No. LA3-H82Fb) with a linear range of 0.1-3 ng/mL (QC tested).

Biotinylated Human LAG-3, Fc,AvitagBiotinylated Human LAG-3, Fc,Avitag (Cat. No. LA3-H82Fb) ELISA bioactivity

Serial dilutions of Anti-LAG3, Neutralizing Antibody were added into Human FGL1, His Tag (Cat. No. FG1-H52Hy): Biotinylated Human LAG-3, Fc,Avitag (Cat. No. LA3-H82Fb) binding reactions. The half maximal inhibitory concentration (IC50) is 0.16695 μg/mL (Routinely tested).

  • Background
    Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, which belongs to immunoglobulin (Ig) superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. LAG3 /CD223 involved in lymphocyte activation. LAG3 /CD223 binds to HLA class-II antigens.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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