Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 75.0 kDa. The protein migrates as 90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human LAG-3, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human LAG-3, Fc,Avitag (Cat. No. LA3-H82Fb) is more than 90% and the molecular weight of this protein is around 200-260 kDa verified by SEC-MALS.
Immobilized Anti-Human LAG3 MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human LAG-3, Fc,Avitag (Cat. No. LA3-H82Fb) with a linear range of 0.1-3 ng/mL (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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