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Product Details
Synonyms
LAG3, CD223, FDC
Source
Biotinylated Human LAG-3, Fc,Avitag (LA3-H82Fb) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1).
Predicted N-terminus: Leu 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 75.0 kDa. The protein migrates as 90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human LAG-3, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human LAG-3, Fc,Avitag (Cat. No. LA3-H82Fb) is more than 95% and the molecular weight of this protein is around 200-260 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Anti-Human LAG3 MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human LAG-3, Fc,Avitag (Cat. No. LA3-H82Fb) with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Serial dilutions of Anti-LAG3, Neutralizing Antibody were added into Human FGL1, His Tag (Cat. No. FG1-H52Hy): Biotinylated Human LAG-3, Fc,Avitag (Cat. No. LA3-H82Fb) binding reactions. The half maximal inhibitory concentration (IC50) is 0.16695 μg/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Lymphocyte activation gene 3 protein
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
32 Details
- Highest Development Stage：
Approved
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