0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > All Other Proteins > BCMA > BCA-HF2H1

FITC-Labeled Human BCMA / TNFRSF17 Protein, His Tag

  • Synonym
    TNFRSF17,CD269,BCM,BCMA
  • Source
    FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H1) is expressed from human HEK293 cells. It contains AA Met 1 - Ala 54 (Accession # Q02223-1). It is the FITC labeled form of Human BCMA, His Tag (Cat. No. BCA-H522y).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    Online(Met 1 - Ala 54) Q02223-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 7.8 kDa. The protein migrates as 10 kDa and 14-16 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
  • FITC:Protein Ratio
    The FITC to protein molar ratio is 1-3.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H1) SDS-PAGE gel

FITC-Labeled Human BCMA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Evaluation of CAR expression
FACS Analysis of Anti-BCMA CAR Expression
Evaluation of CAR expression

293 cells were transfected with anti-BCMA-scFv and RFP tag. 2e5 of the cells were stained with B. FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H1, 1 µg/ml) and C. FITC-labeled protein control. A. Non-transfected 293 cells and C. FITC-labeled protein control were used as negative control. RFP was used to evaluate CAR (anti-BCMA-scFv) expression and FITC was used to evaluate the binding activity of FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H1) (QC tested).

Comments (0)

Order Details

Price(USD) : $319.00

Price(USD) : $1980.00

Promotion & Exhibitions

  • PEGS Boston 2019
  • PepTalk 2019

Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:28 Details
  • Latest Research Phase:Phase ?

Datasheet & Documentation

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Questions & Comments

This web search service is supported by Google Inc.

totop