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Your Position: Home > Cytokines > IL-4 R alpha > ILR-H5253

Human IL-4 R alpha / CD124 Protein, Fc Tag (MALS verified)

  • Synonym
    IL4R,CD124,IL4RA
  • Source
    Human IL-4 R alpha, Fc Tag (ILR-H5253) is expressed from human 293 cells (HEK293). It contains AA Met 26 - His 232 (Accession # P24394-1).
    Predicted N-terminus: Met 26
  • Molecular Characterization
    Online(Met 26 - His 232) P24394-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 50.4 kDa. The protein migrates as 57-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253) SDS-PAGE gel

Human IL-4 R alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human IL-4 R alpha, Fc Tag (Cat. No. ) MALS images

The purity of Human IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253) is more than 90% and the molecular weight of this protein is around 113-138 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human IL-4 R alpha, Fc TagHuman IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253) ELISA bioactivity

Immobilized Human IL-4, Tag Free (Cat. No. IL4-H4218) at 5 μg/mL (100 μL/well)can bind Human IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253) with a linear range of 1-20 ng/mL (QC tested).

Human IL-4 R alpha, Fc TagHuman IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253) ELISA bioactivity

Immobilized Human IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253) at 5 μg/mL (100 μL/well)can bind Biotinylated Human IL-4, Avitag,His Tag (Cat. No. IL4-H82E0) with a linear range of 0.1-2 ng/mL (Routinely tested).

Human IL-4 R alpha, Fc TagHuman IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253) ELISA bioactivity

Serial dilutions of Monoclonal IL-4R/CD124 Neutralizing Antibody were added into Human IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253): Biotinylated Human IL-4, Avitag,His Tag (Cat. No. IL4-H82E0) binding reactions. The half maximal inhibitory concentration (IC50) is 1.144 μg/mL (Routinely tested).

  • Background
    IL-4 is a pleiotropic cytokine produced by activated Th2 cells and mast cells, and plays a pivotal role in immune responses. The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells. Hematopoietic cellular responses to IL-4 are mediated by a high affinity receptor complex comprised of the 140 kDa IL4Rα (CD124)subunit and the 70 kDa common cytokine γc chain (CD132). Interleukin 4 Receptor (IL4R) also known as CD124, IL4Rα and BSF receptor, is a type I cytokine receptor produced by activated Th2 cells and mast cells, and plays an important role in Th2-­biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. A soluble form of the encoded IL4R protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. IL4R can alternatively associate with IL­13Ra1 to form the type II receptor which is responsive to both IL­4 and IL­13. Interleukin-4 receptor has been shown to interact with SHC1.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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