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Your Position: Home > Protein > IL-4 R alpha > ILR-H5253

Human IL-4 R alpha / CD124 Protein, Fc Tag (MALS verified)

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  • Synonym
    IL4R,CD124,IL4RA
  • Source
    Human IL-4 R alpha, Fc Tag(ILR-H5253) is expressed from human 293 cells (HEK293). It contains AA Met 26 - His 232 (Accession # P24394-1).
    Predicted N-terminus: Met 26
  • Molecular Characterization
    IL-4 R alpha Structure

    This protein carries a human IgG1 Fc tag at the C-terminus

    The protein has a calculated MW of 50.4 kDa. The protein migrates as 57-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-4 R alpha SDS-PAGE

Human IL-4 R alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
IL-4 R alpha MALS images

The purity of Human IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253) is more than 90% and the molecular weight of this protein is around 113-138 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IL-4 R alpha ELISA

Immobilized Human IL-4, premium grade (Cat. No. IL4-H4218) at 5 μg/mL (100 μL/well)can bind Human IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253) with a linear range of 1-20 ng/mL (QC tested).

 IL-4 R alpha ELISA

Immobilized Human IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253) at 5 μg/mL (100 μL/well)can bind Biotinylated Human IL-4, Avitag,His Tag (Cat. No. IL4-H82E0) with a linear range of 0.1-2 ng/mL (Routinely tested).

 IL-4 R alpha ELISA

Serial dilutions of Monoclonal IL-4R/CD124 Neutralizing Antibody were added into Human IL-4 R alpha, Fc Tag (Cat. No. ILR-H5253): Biotinylated Human IL-4, Avitag,His Tag (Cat. No. IL4-H82E0) binding reactions. The half maximal inhibitory concentration (IC50) is 1.144 μg/mL (Routinely tested).

  • Background
    IL-4 is a pleiotropic cytokine produced by activated Th2 cells and mast cells, and plays a pivotal role in immune responses. The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells. Hematopoietic cellular responses to IL-4 are mediated by a high affinity receptor complex comprised of the 140 kDa IL4Rα (CD124)subunit and the 70 kDa common cytokine γc chain (CD132).
    Interleukin 4 Receptor (IL4R) also known as CD124, IL4Rα and BSF receptor, is a type I cytokine receptor produced by activated Th2 cells and mast cells, and plays an important role in Th2-­biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. A soluble form of the encoded IL4R protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. IL4R can alternatively associate with IL­13Ra1 to form the type II receptor which is responsive to both IL­4 and IL­13. Interleukin-4 receptor has been shown to interact with SHC1.
  • Clinical and Translational Updates

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