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Your Position: Home > Immune Checkpoint Proteins > B7-H7 > B77-H5257

Human B7-H7 / HHLA2 Protein, Fc Tag (MALS verified)

  • Synonym
    B7-H7,HHLA2,B7 Homolog 7
  • Source
    Human B7-H7, Fc Tag (B77-H5257) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Asn 344 (Accession # Q9UM44-1).
    Predicted N-terminus: Ile 23
  • Molecular Characterization
    Online(Ile 23 - Asn 344) Q9UM44-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 63.4 kDa. The protein migrates as 80-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by reduced SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human B7-H7, Fc Tag (Cat. No. B77-H5257) SDS-PAGE gel

Human B7-H7, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human B7-H7, Fc Tag (Cat. No. ) MALS images

The purity of Human B7-H7, Fc Tag (Cat. No. B77-H5257) was more than 90% and the molecular weight of this protein is around 143-175 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human B7-H7, Fc TagHuman B7-H7, Fc Tag (Cat. No. B77-H5257) ELISA bioactivity

Immobilized Human CD28H, His Tag (Cat. No. CDH-H52H3) at 5 μg/mL (100 μL/well) can bind Human B7-H7, Fc Tag (Cat. No. B77-H5257) with a linear range of 1-19 ng/mL (QC tested).

  • Background
    B7-H7 (HHLA2) is a newly identified B7 family member that regulates human T-cell functions. B7-H7 was previously known as human endogenous retrovirus-H long terminal repeat associating 2 (HHLA2) with unidentified function. Recently, B7-H7 has been identified as a specific ligand for human CD28H. The B7-H7-CD28H pathway strongly promoted CD4+ T-cell proliferation and cytokine production via an AKT-dependent signaling cascade in the presence of TCR signaling, suggesting B7-H7 comprises a new co-stimulatory pathway. The first IgV domain of B7-H7, which presumably binds to a putative receptor, shows the highest homology to other B7 family members.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $300.00

Price(USD) : $1980.00

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