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Product Details
Source
Monoclonal Anti-Adalimumab Antibody, Mouse IgG1 (AY19) (Cat. No. ADB-Y19) is a mouse monoclonal antibody produced from a hybridoma generated by fusion of SP2/0 myeloma cells with mouse B lymphocytes.
Antibody Type
Hybridoma Monoclonal
Clone
AY19
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Immunogen
Adalimumab
Specificity
Recognizes Adalimumab specifically.
Application
ApplicationRecommended Use / PerformanceELISA0.2-2 μg/mLPurification
Protein A purified.
Aggregation
Less than 5%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in Tris (pH 7.5) containing 100 mM Glycine, 25 mM Arginine and 150 mM NaCl, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA
Anti-Adalimumab Antibodies bridging ELISA for Anti-Drug Antibody (ADA) assay development.Immobilized adalimumab at 1 µg/mL, add increasing concentrations of Anti-Adalimumab Antibody (AY19) (Cat. No. ADB-Y19, 10% human serum) and then add biotinylated adalimumab at 5 µg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 0.6 ng/mL (QC tested).
Protocol
Demonstration of the specificity of Anti-Adalimumab Antibody (AY19) (Cat. No. ADB-Y19) to the adalimumab.
Protocol
Measured by its neutralizing ability in a functional ELISA. Immobilized adalimumab at 0.5 μg/mL (100 μL/well) can bind pre-mixed Anti-Adalimumab Antibody (AY19) (Cat. No. ADB-Y19) and Biotinylated Human TNF-alpha, His,Avitag (Cat. No. TNA-H82E3) with an inhibition rate of 100%.
Protocol
Bioactivity-SPR
Anti-Adalimumab Antibody (AY19) (mouse IgG1, Cat. No. ADB-Y19) captured on CM5 chip via anti-mouse antibodies surface, can bind human adalimumab with an affinity constant of 1.36 pM.
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
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Background
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