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Your Position: Home > Antibody > Adalimumab > ADB-Y19

Anti-Adalimumab Antibody (AY19)

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  • Source
    Mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Adalimumab F(ab')2.
  • Isotype
    Mouse IgG1/kappa
  • Specificity
    Recognizes Adalimumab specifically, no cross reactivity with other humanized antibodies.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
Adalimumab SDS-PAGE

Anti-Adalimumab Antibody (AY19) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 Adalimumab ELISA

Anti-Adalimumab Antibodies bridging ELISA for Anti-Drug Antibody (ADA) assay development.Immobilized adalimumab at 1 µg/mL, add increasing concentrations of Anti-Adalimumab Antibody (AY19) (Cat. No. ADB-Y19, 10% human serum) and then add biotinylated adalimumab at 5 µg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 0.6 ng/mL.

 Adalimumab ELISA

Demonstration of the specificity of Anti-Adalimumab Antibody (AY19) (Cat. No. ADB-Y19) to the adalimumab.

 Adalimumab ELISA

Measured by its neutralizing ability in a functional ELISA. Immobilized adalimumab at 0.5 μg/mL (100 μL/well) can bind pre-mixed Anti-Adalimumab Antibody (AY19) (Cat. No. ADB-Y19) and Biotinylated Human TNF-alpha, His,Avitag (Cat. No. TNA-H82E3) with an inhibition rate of 100%.

 Adalimumab SPR

Anti-Adalimumab Antibody (AY19) (mouse IgG1, Cat. No. ADB-Y19) captured on CM5 chip via anti-mouse antibodies surface, can bind human adalimumab with an affinity constant of 1.36 pM.

  • Background
    Adalimumab is a human monoclonal antibody against TNF-alpha. It is produced by recombinant DNA technology using a mammalian cell expression system. It consists of 1330 amino acids and has a molecular weight of approximately 148 kilodaltons.
  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $300.00

Price(USD) : $2180.00

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