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Product Details
Synonyms
IL2, TCGF, lymphokine, Interleukin 2
Source
Biotinylated Human IL-2, Fc,Avitag (IL2-H82F3) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153 (Accession # P60568-1).
Predicted N-terminus: Pro
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the N-terminus and an Avi tag (Avitag™) at the C-terminus.
The protein has a calculated MW of 43.7 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated Human IL-2, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human IL-2, Fc,Avitag (Cat. No. IL2-H82F3) is more than 90% and the molecular weight of this protein is around 80-100 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human IL-2, Fc,Avitag (Cat. No. IL2-H82F3) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) with a linear range of 1-39 ng/mL (QC tested).
Protocol
Immobilized Human IL-2 R alpha, Fc Tag (Cat. No. ILA-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2, Fc,Avitag (Cat. No. IL2-H82F3) with a linear range of 0.6-10 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human IL-2, Fc,Avitag (Cat. No. IL2-H82F3) on SA Biosensor, can bind Human IL-2 R beta, His Tag (SPR verified) (Cat. No. CD2-H5221) with an affinity constant of 2.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Human IL-2, Fc,Avitag (Cat. No. IL2-H82F3) on SA Biosensor, can bind Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) with an affinity constant of 47.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-2
- Category：
- Approved Drugs：
2 Details
- Drugs in Clinical Trials：
46 Details
- Highest Development Stage：
Approved
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