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Your Position: Home > All Other Proteins > Growth Hormone R > GHR-C5253

Rhesus macaque Growth Hormone R (GHR) Protein, Fc Tag

  • Synonym
    GHR,GHBP,GH receptor
  • Source
    Rhesus macaque GHR, Fc Tag (GHR-C5253) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Tyr 264 (Accession # P79194-1).
    Predicted N-terminus: Phe 19
  • Molecular Characterization
    Online(Phe 19 - Tyr 264) P79194-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 54.7 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Rhesus macaque GHR, Fc Tag (Cat. No. GHR-C5253) SDS-PAGE gel

Rhesus macaque GHR, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Rhesus macaque GHR, Fc TagRhesus macaque GHR, Fc Tag (Cat. No. GHR-C5253) ELISA bioactivity

Immobilized Human GH, Tag Free at 2 μg/mL (100 μL/well) can bind Rhesus macaque GHR, Fc Tag (Cat. No. GHR-C5253) with a linear range of 0.039-0.625 μg/mL (QC tested).

  • Background
    Growth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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