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Product Details
Synonyms
CD33 antigen-like 3, SIGLEC-15, CD33L3, sialic acid-binding Ig-like lectin 15, Siglec15, Siglec-15
Source
Human Siglec-15, Fc Tag (SG5-H5253) is expressed from human 293 cells (HEK293). It contains AA Phe 20 - Thr 263 (Accession # Q6ZMC9-1).
Predicted N-terminus: Phe 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 53.0 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human Siglec-15, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) at 5 μg/mL (100 μL/well) can bind Mouse Human chimeric MAb (5G12, Human IgG1) with a linear range of 0.4-6 ng/mL (QC tested).
Protocol
Immobilized Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) at 10 μg/mL (100 μL/well) on Diamond Protein A Protein, His Tag precoated (0.5 μg/well) plate, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with a linear range of 0.078-1.25 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Immobilized Neu5Ac(a2-6)GalNAc-PAA-biotin on SA Chip can bind Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) with an affinity constant of 4.49 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) on ProteinA Biosensor, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with an affinity constant of 0.78 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Anti-Siglec-15 MAb (Mouse IgG) on AMC Biosensor, can bind Human Siglec-15, Fc Tag (Cat. No.SG5-H5253) with an affinity constant of 59.7 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-CELL BASE
Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) inhibits Anti-CD3-induced proliferation of PBMC. The ED50 for this effect is 0.46-0.54 μg/mL (Routinely tested).
Protocol
Customer Reviews Writing Reviews
1***22019-10-12
LikesWe can obtain a good inhibitory effect using this protein to verify its inhibitory on T-cells functions. S15-hFc protein can effectively inhibit the secretion of IFN-gamma stimulated by OKT3 in CD4 + T cells.
Background
Recent Advances
Drug Development Progress- English Name：
Sialic acid-binding Ig-like lectin 15
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
3 Details
- Highest Development Stage：
Phase 2 Clinical
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