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Your Position: Home > All Other Proteins > Siglec-15 > Human Siglec-15 / CD33L3 Protein, Fc Tag

Human Siglec-15 / CD33L3 Protein, Fc Tag

  • Synonym
    CD33 antigen-like 3,SIGLEC-15,CD33L3,sialic acid-binding Ig-like lectin 15,Siglec15,Siglec-15
  • Source
    Human Siglec-15, Fc Tag (SG5-H5253) is expressed from human 293 cells (HEK293). It contains AA Phe 20 - Thr 263 (Accession # Q6ZMC9-1).
    Predicted N-terminus: Phe 20
  • Molecular Characterization
    Online(Phe 20 - Thr 263) Q6ZMC9-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 53.0 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) SDS-PAGE gel

Human Siglec-15, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human Siglec-15, Fc TagHuman Siglec-15, Fc Tag (Cat. No. SG5-H5253) ELISA bioactivity

Immobilized Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) at 5 μg/mL (100 μL/well) can bind Mouse Human chimeric MAb (5G12, Human IgG1) with a linear range of 0.4-6 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Immobilized Neu5Ac(a2-6)GalNAc-PAA-biotin on SA Chip can bind Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) with an affinity constant of 4.49 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) on ProteinA Biosensor, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with an affinity constant of 0.78 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Anti-Siglec-15 MAb (Mouse IgG) on AMC Biosensor, can bind Human Siglec-15, Fc Tag (Cat. No.SG5-H5253) with an affinity constant of 59.7 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Human Siglec-15, Fc Tag (Cat. No. SG5-H5253) inhibits Anti-CD3-induced proliferation of PBMC. The ED50 for this effect is 0.46-0.54 μg/mL (Routinely tested).

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Price(USD) : $350.00

Price(USD) : $2310.00

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