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Your Position: Home > All Other Proteins > Siglec-15 > SG5-H52H3

Human Siglec-15 / CD33L3 Protein, His Tag (MALS verified)

  • Synonym
    CD33 antigen-like 3,SIGLEC-15,CD33L3,sialic acid-binding Ig-like lectin 15,Siglec15,Siglec-15
  • Source
    Human Siglec-15, His Tag (SG5-H52H3) is expressed from human 293 cells (HEK293). It contains AA Phe 20 - Thr 263 (Accession # Q6ZMC9-1).
    Predicted N-terminus: Phe 20
  • Molecular Characterization
    Online(Phe 20 - Thr 263) Q6ZMC9-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 28.4 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Human Siglec-15, His Tag (Cat. No. SG5-H52H3) SDS-PAGE gel

Human Siglec-15, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human Siglec-15, His Tag (Cat. No. ) MALS images

The purity of Human Siglec-15, His Tag(Cat. No. SG5-H52H3) was more than 85% and the molecular weight of this protein is around 60-70 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human Siglec-15, His TagHuman Siglec-15, His Tag (Cat. No. SG5-H52H3) ELISA bioactivity

Immobilized Human Siglec-15, His Tag (Cat. No. SG5-H52H3) at 1 μg/mL (100 μL/well) can bind Mouse Human chimeric mAb (5G12, Human IgG1) with a linear range of 0.2-2 ng/mL (QC tested).

Human Siglec-15, His TagHuman Siglec-15, His Tag (Cat. No. SG5-H52H3) ELISA bioactivity

Immobilized Human Siglec-15, His Tag (Cat. No. SG5-H52H3) at 10 μg/mL (100 μL/well) can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with a linear range of 0.078-0.313 μg/mL (Routinely tested).

  • Background
    Siglec-15 is a DAP12-associated immunoreceptor, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Siglecs are cell surface proteins that bind sialic acid. They are found primarily on the surface of immune cells and are a subset of the I-type lectins. Siglec-15 consisting of immunoglobulin (Ig)-like domains, transmembrane domain and a short cytoplasmic tail. Siglec-15 is that recognizes sialylated glycans and regulates osteoclast differentiation. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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