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Your Position: Home > All Other Proteins > Complement Factor D > CFD-H52H8

Human Complement Factor D / CFD Protein, His Tag (active enzyme)

  • Synonym
    CFD,Adipsin,PFD,DF,Complement factor D
  • Source
    Human Complement Factor D, His Tag (CFD-H52H8) is expressed from human 293 cells (HEK293) and active. It contains AA Ile 26 - Ala 253 (Accession # P00746-1).
    Predicted N-terminus: Ile 26
  • Molecular Characterization
    Online(Ile 26 - Ala 253) P00746-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 26.3 kDa. The protein migrates as 28 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0. Normally glycerol is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Complement Factor D, His Tag (Cat. No. CFD-H52H8) SDS-PAGE gel

Human Complement Factor D, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is >80 pmol/min/μg (QC tested).

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Datasheet & Documentation

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DMF (Drug Master File)

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