RFP Fusion Human Mesothelin / MSLN (296-580) Protein, His Tag

Related Products: Cell line CHEK-ATP119
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MSN-HR2P4-25ug
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  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
  • Synonyms

    MSLN, Mesothelin, MPF

  • Source

    RFP Fusion Human Mesothelin (296-580), His Tag (MSN-HR2P4) is expressed from human 293 cells (HEK293). It contains AA Glu 296-Gly 580 (Accession # AAH09272.1 ).

    Predicted N-terminus: Glu 296

    Request for sequence
  • Molecular Characterization

    Mesothelin Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 62 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    RFP

    Excitation source: 561 nm spectral line, Yellow Green Laser

    Excitation Wavelength: 561 nm

    Emission Wavelength: 586/15 nm

  • Application

    Flow cytometry (E.g. Evaluation of anti-MSLN CAR expression), fluorescence microscopy, etc.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

Background

Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.