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Your Position: Home > Immune Checkpoint Proteins > ICOS > Human ICOS / CD278 Protein, Fc Tag

Human ICOS / CD278 Protein, Fc Tag

  • Synonym
    ICOS,CD278,AILIM,Inducible T-cell costimulator
  • Source
    Human ICOS, Fc Tag (ICS-H5258) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Phe 141 (Accession # Q9Y6W8-1).
    Predicted N-terminus: Glu 21
  • Molecular Characterization
    Online(Glu 21 - Phe 141) Q9Y6W8-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 40.2 kDa. The protein migrates as 46-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ICOS, Fc Tag (Cat. No. ICS-H5258) SDS-PAGE gel

Human ICOS, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human ICOS, Fc TagHuman ICOS, Fc Tag (Cat. No. ICS-H5258) ELISA bioactivity

Immobilized Human B7-H2, His Tag (Cat. No. B72-H5221) at 1 μg/mL (100 μL/well) can bind Human ICOS, Fc Tag (Cat. No. ICS-H5258) with a linear range of 0.4-6 ng/mL (QC tested).

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Price(USD) : $350.00

Price(USD) : $2310.00

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