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Your Position: Home > Cytokines > EGF > EGF-M5265

Mouse EGF Protein, Fc Tag

  • Synonym
    EGF,URG,HOMG4
  • Source
    Mouse EGF, Fc Tag (EGF-M5265) is expressed from human 293 cells (HEK293). It contains AA Asn 977 - Arg 1029 (Accession # P01132-1).
    Predicted N-terminus: Pro
  • Molecular Characterization
    Online(Asn 977 - Arg 1029) P01132-1

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 32.5 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse EGF, Fc Tag (Cat. No. EGF-M5265) SDS-PAGE gel

Mouse EGF, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Mouse EGF, Fc TagMouse EGF, Fc Tag (Cat. No. EGF-M5265) ELISA bioactivity

Immobilized Mouse EGF R, His Tag (Cat. No. EGR-M5224) at 5 μg/mL (100 μL/well) can bind Mouse EGF, Fc Tag (Cat. No. EGF-M5265) with a linear range of 0.2-5 ng/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Captured Mouse EGF R, His Tag (Cat. No. EGR-M5224) on CM5 Chip via anti-His antibody can bind Mouse EGF, Fc Tag (Cat. No. EGF-M5265) with an affinity constant of 5.44 nM as determined in SPR assay (Biacore T200 ) (Routinely tested).

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Price(USD) : $290.00

Price(USD) : $1910.00

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