>Integrin alpha V beta 8
Mouse ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 112.5 kDa (ITGAV) and 77.5 kDa (ITGB8). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-150 kDa (ITGAV) and 80-90 kDa (ITGB8) respectively due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mouse ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Mouse ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT8-M52W6) is more than 85% and the molecular weight of this protein is around 189-231 kDa verified by SEC-MALS.
Immobilized Mouse ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT8-M52W6) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGF-Beta 1, His,Avitag (Cat. No. TG1-H82Qb) with a linear range of 1-10 ng/mL (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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