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Your Position: Home > Immune Checkpoint Proteins > SLAMF7 > SL7-HF2H7

FITC-Labeled Human SLAMF7 / CRACC / CD319 Protein, His Tag

  • Synonym
    SLAMF7,CD319,CS1,CRACC,19A,FOAP-12
  • Source
    FITC-Labeled Human SLAMF7, His Tag (Cat. No. SL7-HF2H7) is expressed from human HEK293 cells. It contains AA Ser 23 - Met 226 (Accession # AAH27867). It is the FITC labeled form of Human SLAMF7, His Tag (Cat. No. SL7-H5225).
    Predicted N-terminus: Ser 23
  • Molecular Characterization
    Online(Ser 23 - Met 226) AAH27867

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 23.2 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
  • FITC:Protein Ratio
    The FITC to protein molar ratio is 3-4.5.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FITC-Labeled Human SLAMF7, His Tag (Cat. No. SL7-HF2H7) SDS-PAGE gel

FITC-Labeled Human SLAMF7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of Anti-SLAMF7 CAR-293 cells were stained with 100 μL of 10 μg/mL of FITC-Labeled Human SLAMF7, His Tag (Cat. No. SL7-HF2H7) and negative control protein respectively, FITC signal was used to evaluate the binding activity (QC tested)

  • Background
    SLAM family member 7 (SLAMF7) is also known as CD2-like receptor-activating cytotoxic cells (CRACC), Membrane protein FOAP-12, CD antigen CD319, Novel Ly9, Protein 19A, which is a single-pass type I membrane protein and a member of the CD2 family of cell surface receptors. SLAMF7 is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Isoform 1 of SLAMF7 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway. May play a role in lymphocyte adhesion. Isoform 3 of SLAMF7 does not mediate any NK cell activation.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $319.00

Price(USD) : $1980.00

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  • Number of Drugs in Clinical Trials:3 Details
  • Latest Research Phase:Approved

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