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Your Position: Home > All Other Proteins > CD3E & CD3D > CDD-C5259

Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin

  • Synonym
    CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta / epsilon
  • Source
    Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin (CDD-C5259) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # Q95LI5-1 (CD3E) & Q95LI8-1 (CD3D)).
    Predicted N-terminus: Gln 22 (CD3E) & Phe 22 (CD3D)
  • Molecular Characterization
    Online(Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D)) Q95LI5-1 (CD3E) & Q95LI8-1 (CD3D)

    Cynomolgus CD3E & CD3D Heterodimer Protein, Llama IgG2b Fc Tag & Llama IgG2b Fc Tag, low endotoxin is produced by co-expression of CD3E and CD3D, has a calculated MW of 41.9 kDa (CD3E) and 40.8 kDa (CD3D). Subunit CD3E is fused with a llama IgG2b Fc tag at the C-terminus and subunit CD3D is fused with a llama IgG2b Fc tag at the C-terminus. The predicted N-terminus is Gln 22 (CD3E) & Phe 22 (CD3D). The reducing (R) protein migrates as 50 kDa and 55-65 kDa due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >90% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin (Cat. No. CDD-C5259) SDS-PAGE gel

Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxinCynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin (Cat. No. CDD-C5259) ELISA bioactivity

Immobilized Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin (Cat. No. CDD-C5259) at 2 μg/mL (100 μL/well) can bind Mouse Anti-Human CD3 (SP34-2) (mouse IgG1) with a linear range of 1-20 ng/mL (QC tested).

Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.

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Price(USD) : $350.00

Price(USD) : $2310.00

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