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Your Position: Home > All Other Proteins > CD3E & CD3D > CDD-H5258

Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin

  • Synonym
    CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta / epsilon
  • Source
    Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin (CDD-H5258) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D)).
    Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)
  • Molecular Characterization
    Online(Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D)) P07766-1 (CD3E) & P04234-1 (CD3D)

    Human CD3E & CD3D Heterodimer Protein, Llama IgG2b Fc Tag & Llama IgG2b Fc Tag, low endotoxin is produced by co-expression of CD3E and CD3D, has a calculated MW of 42.9 kDa (CD3E) and 40.8 kDa (CD3D). Subunit CD3E is fused with a llama IgG2b Fc tag at the C-terminus and subunit CD3D is fused with a llama IgG2b Fc tag at the C-terminus. The predicted N-terminus is Asp 23 (CD3E) & Phe 22 (CD3D). The reducing (R) protein migrates as 50-60 kDa due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin (Cat. No. CDD-H5258) SDS-PAGE gel

Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxinHuman CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin (Cat. No. CDD-H5258) ELISA bioactivity

Immobilized Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin (Cat. No. CDD-H5258) at 2 μg/mL (100 μL/well) can bind SP34-2 (mouse IgG1) with a linear range of 1-20 ng/mL (Routinely tested).

Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.

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Price(USD) : $350.00

Price(USD) : $2310.00

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