This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 22.8 kDa. The protein migrates as 28-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mouse CD16-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Mouse CD16-2, His Tag(Cat. No. FC4-M52H3) was more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.
Captured Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) on NTA-Series S sensor chip can bind OKT3 with an affinity constant of 154 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) on NTA Biosensor, can bind OKT3 with an affinity constant of 140 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Mouse CD16-2, His Tag (Cat. No. FC4-M52H3) on NTA Biosensor, can bind Herceptin with an affinity constant of 122 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Chini CCS, Peclat TR, Warner GM, et al.
Journal: Nature metabolism 2020
Application: Binding Assay
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