This protein carries a mouse IgG2a Fc tag at the C-terminus
The protein has a calculated MW of 57.0 kDa. The protein migrates as 75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human CD19 (20-291), Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human CD19 (20-291), Mouse IgG2a Fc Tag (Cat. No. CD9-H5258) is more than 90% and the molecular weight of this protein is around 130-150 kDa verified by SEC-MALS.
Immobilized Human CD19 (20-291), Mouse IgG2a Fc Tag (Cat. No. CD9-H5258) at 5 μg/mL (100 μL/well) can bind Mouse / Human chimeric FMC63 MAb (human IgG1) with a linear range of 0.5-8 ng/mL (QC tested).
Loaded Mouse / Human chimeric FMC63 MAb (human IgG1) on AHC Biosensor, can bind Human CD19 (20-291), Mouse IgG2a Fc Tag (Cat. No. CD9-H5258) with an affinity constant of 0.487 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $410.00
Price(USD) : $2640.00
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