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Product Details
Synonyms
TACSTD2, GA733-1, M1S1, TROP2
Source
Biotinylated Human TROP-2, His,Avitag (TR2-H82E5) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Thr 274 (Accession # P09758-1).
Predicted N-terminus: Gln 31
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 31.2 kDa. The protein migrates as 42-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human TROP-2, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human TROP-2, His,Avitag (Cat. No. TR2-H82E5) is more than 95% and the molecular weight of this protein is around 32-47 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human TROP-2, His,Avitag (Cat. No. TR2-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse Monoclonal Antibody Against Human TROP-2, Mouse IgG1 with a linear range of 0.5-4 ng/mL (QC tested).
Protocol
Bioactivity-TR-FRET
In this TR-FRET assay, Streptavidin Protein Europium chelate (for TR-FRET) (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-Human IgG Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA02) is used as the Acceptor. Biotinylated Human TROP-2, His,Avitag (Cat. No. TR2-H82E5), at 0.3125 ng/well, can bind to the Sacituzumab with a linear detection range of 4-0.001 μg/mL (Routinely tested).
Bioactivity-SPR
Captured Trop2 antibody on CM5 chip via anti-mouse antibodies surface can bind Biotinylated Human TROP-2, His,Avitag (Cat. No. TR2-H82E5) with an affinity constant of 6.35 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor-associated calcium signal transducer 2
- Category：
- Approved Drugs：
3 Details
- Drugs in Clinical Trials：
47 Details
- Highest Development Stage：
Approved
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