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Product Details
Source
Anti-Cetuximab Antibody (AY27) (Cat. No. CEB-Y27) is a mouse monoclonal antibody produced from a hybridoma generated by fusion of SP2/0 myeloma cells with mouse B lymphocytes.
Antibody Type
Hybridoma Monoclonal
Clone
AY27
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Immunogen
Cetuximab
Specificity
Recognizes Cetuximab specifically.
Application
ApplicationRecommended Use / PerformanceELISA0.2-2 μg/mLPurification
Protein A purified.
Aggregation
Less than 5%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in Tris (pH 7.5) containing 100 mM Glycine, 25 mM Arginine and 150 mM NaCl, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA
Anti-Cetuximab Antibodies bridging ELISA for Anti-Drug Antibody (ADA) assay development. Immobilized cetuximab at 1 µg/mL, add increasing concentrations of Anti-Cetuximab Antibody (AY27) (Cat. No. CEB-Y27, 10% human serum) and then add biotinylated cetuximab at 2 µg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 1.2 ng/mL (QC tested).
Protocol
Demonstration of the specificity of Anti-Cetuximab Antibody (AY27) (Cat. No. CEB-Y27) to the cetuximab.
Protocol
Immobilized cetuximab at 0.2 μg/mL (100 μL/well) can bind pre-mixed Anti-Cetuximab Antibody (AY27) (Cat. No. CEB-Y27) and Biotinylated Human EGFRvIII, His,Avitag (Cat. No. EGR-H82E0) with a inhibition rate of 100%.
Protocol
Serial dilutions of pre-mixed Anti-Cetuximab Antibody (AY27) (Cat. No. CEB-Y27) were added into Monoclonal Anti-EGF R Antibody, Human IgG1, Cetuximab: Biotinylated Human EGF R, His,Avitag (Cat. No. EGR-H82E3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.1496 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Anti-Cetuximab Antibody (AY27) (mouse IgG1, Cat. No. CEB-Y27) captured on CM5 chip via anti-mouse antibodies surface, can bind Human cetuximab with an affinity constant of 7.28 pM.
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
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