Mouse Complement C5 Protein, His Tag

Synonyms

Complement C5, C5, CPAMD4

Source

Mouse Complement C5, His Tag (CO5-M52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Leu 678 & Asn 679 - Glu 1680 (Accession # P06684-1).

Predicted N-terminus: Gln 19 (β chain) & Asn 679 (α chain)

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus.

The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.5 kDa (β chain) and 114.6 kDa (α chain). The protein migrates as 66 kDa (β chain) and 100 kDa (α chain) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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