>Fc gamma RI / CD64
Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 34.8 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Cynomolgus CD64, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Biotinylated Cynomolgus CD64, His,Avitag (Cat. No. FCA-C82E8) is more than 90% and the molecular weight of this protein is around 37-55 kDa verified by SEC-MALS.
Immobilized Ipilimumab at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD64, His,Avitag (Cat. No. FCA-C82E8) with a linear range of 0.1-2 ng/mL (QC tested).
Biotinylated Cynomolgus CD64, His,Avitag (Cat. No. FCA-C82E8) captured on Biotin CAP - Series S sensor Chip can bind Herceptin with an affinity constant of 1.50 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Loaded Biotinylated Cynomolgus CD64, His,Avitag (Cat. No.FCA-C82E8) on SA Biosensor, can bind Herceptin with an affinity constant of 0.326 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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