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Your Position: Home > Immune Checkpoint Proteins > CD47 > Canine CD47 Protein, His Tag

Canine CD47 Protein, His Tag

  • Synonym
    CD47,MER6,IAP,OA3
  • Source
    Canine CD47, His Tag (CD7-C52H5) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Asn 140 (Accession # F1P6D7-1).
    Predicted N-terminus: Gln 19
  • Molecular Characterization
    Online(Gln 19 - Asn 140) F1P6D7-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 15.8 kDa. The protein migrates as 27-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Canine CD47, His Tag (Cat. No. CD7-C52H5) SDS-PAGE gel

Canine CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Canine CD47, His TagCanine CD47, His Tag (Cat. No. CD7-C52H5) ELISA bioactivity

Immobilized Human SIRP alpha, Fc Tag (HPLC-verified) (Cat. No. SIA-H5251) at 5 μg/mL (100 μL/well) can bind Canine CD47, His Tag (Cat. No. CD7-C52H5) with a linear range of 5-78 ng/mL (QC tested).

  • Background
    Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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