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Your Position: Home > Protein > Mesothelin > MSN-HF25x

FITC-Labeled Human Mesothelin / MSLN (296-580) Protein, Fc Tag

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  • Synonym
    MSLN,Mesothelin,MPF
  • Source
    FITC-Labeled Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-HF25x) is expressed from human HEK293 cells. It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1). It is the FITC labeled form of Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H526x).
  • Molecular Characterization
    Mesothelin Structure

    This protein carries a human IgG1 Fc tag at the N-terminus

    The protein has a calculated MW of 59.1 kDa. The protein migrates as 60-67 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.
  • Protein Ratio
    The FITC to protein molar ratio is 1-2.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mesothelin SDS-PAGE

FITC-Labeled Human Mesothelin (296-580), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 Mesothelin ELISA

Immobilized Anti-Human MSLN antibody at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-HF25x) with a linear range of 0.039-0.625 μg/mL (QC tested).

Bioactivity-FACS
 Mesothelin FACS

2e5 of anti MSLN-CAR-293 cells transfected with Anti-MSLN scfv were stained with 100 μL of 3 μg/mL of FITC-Labeled Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-HF25x) and negative control protein respectively, FITC signal was used to evaluate the binding activity (QC tested).

  • Background
    Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.
  • Clinical and Translational Updates

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Price(USD) : $319.00

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