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Your Position: Home > All Other Proteins > LRP-5 > LR5-H5254

Human LRP-5 Protein, Mouse IgG2a Fc Tag

  • Synonym
    LRP5,LRP-5,LRP-7,LRP7,LR3
  • Source
    Human LRP-5, Mouse IgG2a Fc Tag (LR5-H5254) is expressed from human 293 cells (HEK293). It contains AA Glu 644 - Gln 1263 (Accession # O75197-1 ).
    Predicted N-terminus: Glu 644
  • Molecular Characterization
    Online(Glu 644 - Gln 1263) O75197-1 

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 96.6 kDa. The protein migrates as 110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human LRP-5, Mouse IgG2a Fc Tag (Cat. No. LR5-H5254) SDS-PAGE gel

Human LRP-5, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human LRP-5, Mouse IgG2a Fc TagHuman LRP-5, Mouse IgG2a Fc Tag (Cat. No. LR5-H5254) ELISA bioactivity

Immobilized Biotinylated Human Dkk-1, Fc,Avitag (Cat. No. DK1-H82F5) at 5 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Human LRP-5, Mouse IgG2a Fc Tag (Cat. No. LR5-H5254) with a linear range of 0.5-16 ng/mL (QC tested).

Human LRP-5, Mouse IgG2a Fc TagHuman LRP-5, Mouse IgG2a Fc Tag (Cat. No. LR5-H5254) ELISA bioactivity

Immobilized Human Dkk-1, His Tag (Cat. No. DK1-H5221) at 5 μg/mL (100 μL/well) can bind Human LRP-5, Mouse IgG2a Fc Tag (Cat. No. LR5-H5254) with a linear range of 0.01-0.313 μg/mL (Routinely tested).

  • Background
    Low-density lipoprotein receptor-related protein 5(LRP-5) is also known as  BMND1, EVR1, EVR4, HBM, LR3, LRP7, OPPG, OPTA1, VBCH2, LDL receptor related protein 5 and PCLD4. LRP5 is a transmembrane low-density lipoprotein receptor that shares a similar structure with LRP6. LRP5 acts as a co-receptor with LRP6 and the Frizzled protein family members for transducing signals by Wnt proteins through the canonical Wnt pathway. This protein plays a key role in skeletal homeostasis. Mutations in LRP5 can lead to considerable changes in bone mass. A loss-of-function mutation causes osteoporosis-pseudoglioma (decrease in bone mass), while a gain-of-function mutation causes drastic increases in bone mass.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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