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Your Position: Home > All Other Proteins > Siglec-15 > Cynomolgus Siglec-15 / CD33L3 Protein, Fc Tag

Cynomolgus Siglec-15 / CD33L3 Protein, Fc Tag

  • Synonym
    CD33 antigen-like 3,SIGLEC-15,CD33L3,sialic acid-binding Ig-like lectin 15,Siglec15,Siglec-15
  • Source
    Cynomolgus Siglec-15, Fc Tag (SG5-C5253) is expressed from human 293 cells (HEK293). It contains AA Phe 20 - Thr 263 (Accession # A0A2K5UY47-1).
    Predicted N-terminus: Phe 20
  • Molecular Characterization
    Online(Phe 20 - Thr 263) A0A2K5UY47-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 52.7 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus Siglec-15, Fc Tag (Cat. No. SG5-C5253) SDS-PAGE gel

Cynomolgus Siglec-15, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Cynomolgus Siglec-15, Fc TagCynomolgus Siglec-15, Fc Tag (Cat. No. SG5-C5253) ELISA bioactivity

Immobilized Cynomolgus Siglec-15, Fc Tag (Cat. No. SG5-C5253) at 5 μg/mL (100 μL/well) can bind Mouse Human chimeric MAb (5G12, Human IgG1) with a linear range of 0.4-6 ng/mL (QC tested).

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Price(USD) : $350.00

Price(USD) : $2310.00

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