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Your Position: Home > Protein > Glypican 1 > GP1-M52H7

Mouse Glypican 1 / GPC1 Protein, His Tag

  • Synonym
    Glypican 1,GPC1
  • Source
    Mouse Glypican 1, His Tag (GP1-M52H7) is expressed from human 293 cells (HEK293). It contains AA Asp 24 - Ser 529 (Accession # Q9QZF2-1).
    Predicted N-terminus: Asp 24
  • Molecular Characterization
    Online(Asp 24 - Ser 529) Q9QZF2-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 57.5 kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse Glypican 1, His Tag (Cat. No. GP1-M52H7) SDS-PAGE gel

Mouse Glypican 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Mouse Glypican 1, His TagMouse Glypican 1, His Tag (Cat. No. GP1-M52H7) ELISA bioactivity

Immobilized Human FGF basic, premium grade (Cat. No. BFF-H4117) at 1 μg/mL (100 μL/well) can bind Mouse Glypican 1, His Tag (Cat. No. GP1-M52H7) with a linear range of 5-78 ng/mL (QC tested).

  • Background
    Glypican 1 has been shown to interact with SLIT2. This protein is involved in the misfolding of normal prion proteins in the cell membrane to the infectious prion form. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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