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Your Position: Home > Protein > Glypican 3 > GP3-C5259

Cynomolgus Glypican 3 / GPC3 Protein, Llama IgG2b Fc Tag, low endotoxin

  • Synonym
    GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1
  • Source
    Cynomolgus Glypican 3 Protein, Llama IgG2b Fc Tag (GP3-C5259) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # XP_005594665.1).
    Predicted N-terminus: Gln 25 & Ser 359
  • Molecular Characterization
    Online(Gln 25 - His 559) XP_005594665.1

    This protein carries a llama IgG2b Fc tag at the C-terminus. This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 50.5 kDa respectively. The protein migrates as 40 kDa, 60 kDa and 100-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus Glypican 3 Protein, Llama IgG2b Fc Tag (Cat. No. GP3-C5259) SDS-PAGE gel

Cynomolgus Glypican 3 Protein, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Cynomolgus Glypican 3 Protein, Llama IgG2b Fc TagCynomolgus Glypican 3 Protein, Llama IgG2b Fc Tag (Cat. No. GP3-C5259) ELISA bioactivity

Immobilized Human FGF basic, premium grade (Cat. No. BFF-H4117) at 1 μg/mL (100 μL/well) can bind Cynomolgus Glypican 3 Protein, Llama IgG2b Fc Tag (Cat. No. GP3-C5259) with a linear range of 1-63 ng/mL (QC tested).

Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.
  • Background
    Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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