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Your Position: Home > All Other Proteins > Glypican 3 > Human Glypican 3 / GPC3 Protein, Llama IgG2b Fc Tag, low endotoxin

Human Glypican 3 / GPC3 Protein, Llama IgG2b Fc Tag, low endotoxin

  • Synonym
    GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1
  • Source
    Human Glypican 3, Llama IgG2b Fc Tag, low endotoxin (GP3-H5257) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1).
    Predicted N-terminus: Gln 25
  • Molecular Characterization
    Online(Gln 25 - His 559) P51654-1

    This protein carries a llama IgG2b Fc tag at the C-terminus.

    The protein has a calculated MW of 88.6 kDa. The protein migrates as 42 kDa, 60 kDa and 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Glypican 3, Llama IgG2b Fc Tag, low endotoxin (Cat. No. GP3-H5257) SDS-PAGE gel

Human Glypican 3, Llama IgG2b Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human Glypican 3, Llama IgG2b Fc Tag, low endotoxinHuman Glypican 3, Llama IgG2b Fc Tag, low endotoxin (Cat. No. GP3-H5257) ELISA bioactivity

Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 1 μg/mL (100 μL/well) can bind Human Glypican 3, Llama IgG2b Fc Tag, low endotoxin (Cat. No. GP3-H5257) with a linear range of 0.6-39 ng/mL (QC tested).

Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.
  • Background
    Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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