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Product Details
Source
Biotinylated Anti-Cetuximab Antibody (AY31) (Cat. No. CEB-BY31) is a mouse monoclonal antibody produced from a hybridoma generated by fusion of SP2/0 myeloma cells with mouse B lymphocytes.
Antibody Type
Hybridoma Monoclonal
Clone
AY31
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Immunogen
Cetuximab
Conjugate
Biotinylated
Specificity
Recognizes Cetuximab specifically.
Application
ApplicationRecommended Use / PerformanceELISA1:300-1:3000Purification
Protein A purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% PF68, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA
Immobilized Anti-Cetuximab Antibody (12B1E9) (Cat. No.CEB-Y29c) at 2 μg/mL, add increasing concentrations of Cetuximab and then add Biotinylated Anti-Cetuximab Antibody (AY31) (Cat. No.CEB-BY31) at 1 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4.84 ng/mL (QC tested).
Protocol
Immobilized cetuximab at 0.1 μg/mL (100 μL/well) can bind Biotinylated Anti-Cetuximab Antibody (AY31) (Cat. No. CEB-BY31) with a linear range of 0.39-25 ng/mL.
Protocol
ELISA analysis shows that the binding of cetuximab to Human EGF R, His Tag (Cat. No. EGR-H5222) was inhibited by increasing concentration of Biotinylated Anti-Cetuximab Antibody (AY31) (Cat. No. CEB-BY31). The concentration of cetuximab used is 3 ng/mL.
Protocol
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FAQ
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Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background
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