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Your Position: Home > All Other Proteins > CRTAM > Human CRTAM Protein, His Tag

Human CRTAM Protein, His Tag

  • Synonym
    CRTAM,CD355
  • Source
    Human CRTAM, His Tag (CRM-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ser 18 - Gly 287 (Accession # O95727-1).
    Predicted N-terminus: Ser 18
  • Molecular Characterization
    Online(Ser 18 - Gly 287) O95727-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 31.9 kDa. The protein migrates as 42-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CRTAM, His Tag (Cat. No. CRM-H52H3) SDS-PAGE gel

Human CRTAM, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human CRTAM, His Tag (Cat. No. CRM-H52H3) on AR2G Biosensor, can bind Human CADM1, His Tag (Cat. No. CA1-H5225) with an affinity constant of 51.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Class I-restricted T cell-associated molecule (CRTAM), a member of nectin family and the immunoglobulin superfamily, is also known as cytotoxic and regulatory T-cell molecule, which is expressed by activated CD8+ and NK T cells. CRTAM is found in spleen, thymus, small intestine, peripheral blood, and surprisingly, in brain where it is highly expressed by Purkinje cells of the cerebellum. The high affinity of CRTAM/IGSF4 adhesion allows CRTAM to disrupt IGSF4 homotypic interactions (3 - 5). IGSF4 and T cell receptor co-engagement of CRTAM-expressing CD8+ cells induces increased IFN-gamma or IL-22 production (3, 4). Furthermore, a role in cancer surveillance through NK cell-mediated rejection of IGSF4-expressing tumors has been proposed.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $300.00

Price(USD) : $1980.00

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